By Sean R. Eddy (auth.), F. Lamberti, C. De Giorgi, David McK. Bird (eds.)
Plant parasitic nematodes are a major pest to plants. For ex am pie, the root-knot nematodes belonging to the genus Meloidogyne are world wide of their distribution and assault virtually every kind of crop, inflicting significant losses of yield and affecting caliber of produce. The cyst nematodes in the genera Globodera and Heterodera represent a tremendous crew of plant pathogens in lots of international locations in the course of the global, suppressing yields of potato, sugar beet, soybean and cereals. a number of nematodes akin to longidorids and trichodorids are implicated within the transmission of diverse plant viruses. Many others reason constraints to agricultural construction both in the neighborhood or on huge parts. even if, regardless of their fiscal significance (they account for around the globe crop aid in far more than 10%), plant parasitic nematodes are nonetheless poorly understood, simply because so much of them are obligate parasites of roots. Environmental issues over the rural use of insecticides call for the advance of different measures to manage them. to accomplish environmentally sound keep watch over, wisdom of the elemental biology of nematodes needs to be accelerated. very important learn parts comprise knowing the molecular bases for pathogenicity, the molecular mechanisms of the host parasite interactions and the genetic bases for inhabitants fluctuations. The workshop has, for the 1st time, introduced jointly a world crew of researchers utilizing molecular methods to check plant parasitic nematodes and their host responses.
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Extra info for Advances in Molecular Plant Nematology
1992, The meiotic behavior of an inversion in Caenorhabditis elegans, Genetics 131:321. M. A, 1993, Target-selected gene inactivation in Caenorhabditis elegans by using a frozen transposon insertion mutant bank, Proc. Nat!. Acad. Sei USA 90:7431. A. 2078 06606 Antibes Cedex, France INTRODUCTION Transposable elements are present in the genomes of most, if not all, organisms. Because of their ability to insert into and excise from the chromosomes of their hosts transposons are a significant source of spontaneous mutations in organisms.
Examples of eaeh type of transposable element are given. (a,b) class I : transposable elements believed to transpose via RNA intennediates. (e,d) class II : transposable elements believed to transpose directIy from DNA to DNA. (from Finnegan, 1989). genetie studies, This article foeuses prineipally on Tel (Te= transposon of C. , 1992). However, other transposable elements in C. elegans and in other species such as C. briggsae, Ascaris lumbricoides and Panagrellus redivivus have been described. This article also deals with these elements and also the different strategies for cloning transposable elements in species other than the ones described above.
We have included a KanR cassette (Genblock™-Pharmacia) in a plasmid clone of pUC18 (Messing, 1983) in addition to the rol-6 plasmid for co-injection with the Lorist vectors (cosmids beginning with K and T). Lorist vectors confer kanamycin resistance and have little sequence identity with pRF4. Strains segregating a high percentage of roller progeny have been established, used in genetic crosses, and frozen for future experiments. The advantage of establishing strains that transmit the cosmids as extrachromosomal arrays is that these strains can be maintained and used repeatedly in genetic crosses designed to study aspects of the mutant phenotypes that they rescue.
Advances in Molecular Plant Nematology by Sean R. Eddy (auth.), F. Lamberti, C. De Giorgi, David McK. Bird (eds.)